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Conjugatable Phosphorescence (µS) Probes

We offer a unique range of high quality Phosphorescent Dyes for labelling / conjugating to biomolecules.

Methuselah Green Carboxy™

Methuselah Green Carboxy™ is a water soluble probe which can readily be used to prepare an amine-reactive version of Methuselah Green, which is ideal for the labelling of biomolecules, crosslinking, and solid-phase immobilization applications. Methuselah Green Carboxy (-COOH) can be reacted to NHS or Sulfo-NHS in the presence of a carboiimide such as EDC (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide), resulting in a stable NHS or Sulfo-NHS ester form of Methuselah Green, which can then be reacted with primary amines (-NH2) to form amide crosslinks. NHS–(N-hydroxysuccinimide). Sulfo-NHS (N-hydroxysulfosuccinimide).

Both NHS and Sulfo-NHS are soluble in aqueous and organic solvents; however dye activation with Sulfo-NHS preserves or increases water solubility of the modified molecule.

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Figure 1. (A) Absorbance spectra recorded for Methuselah Green Carboxy™ dissolved in glycerol. (B) Same data as shown in panel A but on a different scale. (C) Phosphorescence spectra recorded for Methuselah Green Carboxy™ dissolved in glycerol. The excitation wavelength was 300 nm.

 

Methuselah Green Carboxy™ has similar fluorescence / phosphorescence properties as Methuselah Green 2™, where both the fluorescence and phosphorescence bands of Methuselah Green 2™ can be excited 290-350 nm with phosphorescence emission centered around 530 nm. The phosphorescence lifetime of Methuselah Green Carboxy™ is excitation wavelength dependent, ranging from 1671 ±6 µS at 280 nm, to 1069±16 µS when excited at 350 nm.

Similar to Methuselah Green 2™, Methuselah Green Carboxy™ (-COOH) can be used for any kind of spectroscopy in addition to activating its carboxy group for coupling purposes.

Methuselah Green Carboxy™ is available in both 1 and 5 mg lyophilized vials.

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Figure 2. Phosphorescence decays observed for Methuselah Green Carboxy™ dissolved in glycerol following excitation at different wavelengths. The emission wavelength was kept fix at 550 nm. The table shows fitted decay constants.

 

Methuselah Green Hydroxyl™

Methuselah Green Hydroxyl™ (-OH) is slightly less water soluble than Methuselah Green Carboxy™ (-COOH). Excitation can readily be performed from 290-350 nm, with a phosphorescence emission centered around 530 nm. The phosphorescence lifetime, when excited at 350 nm, is ≈ 229 ± 1 µs.

Methuselah Green Hydroxyl™ is available in both 1 and 5 mg lyophilized vials.

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Figure 3. (A) Absorbance spectra recorded for Methuselah Green Hydroxyl™ dissolved in methanol. (B) Phosphorescence spectra recorded for Methuselah Green Hydroxyl™ dissolved in glycerol exciting at 320 nm. (C) Phosphorescence decay recorded for Methuselah Green Hydroxyl™ dissolved in glycerol exciting at 320 nm and recording the emission at 520 nm. The data was fitted to a mono-exponential decay function (229 ± 1 µs) indicated by the blue line.

 

Phos Hydroxyl™

Phos Hydroxyl™ (-OH) is slightly less water soluble than Phos 2™, with excitation from 250-320 nm, and a phosphorescence emission centered ≈ 535 nm with a lifetime (in glycerol) of 97.1 ±1.1 µS.

Phos Hydroxyl™ is available in both 1 and 5 mg lyophilized vials.

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Figure 4. (A) Absorption spectra recorded for Phos Hydroxyl™ dissolved in methanol. (B) Phosphorescence spectra recorded for Phos Hydroxyl™ dissolved in glycerol exciting at 300 nm. (C) Phosphorescence decay recorded for Phos Hydroxyl™ dissolved in glycerol exciting at 300 nm and recording the emission at 520 nm. The data was fitted to a mono-exponential decay function (97.1±1.1 us) indicated by the blue line.

 

 

MethuselahGreenCarboxy

Methuselah Green Carboxy™ is a water soluble phosphorescent probe which can readily be used for the labelling of biomolecules, crosslinking, and solid-phase immobilization applications. The phosphorescence decay constant for Methuselah Green Carboxy™ is in the ms time domain.