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Glucose Sensing

Ursa BioScience offers a patent protected unique range of highly sensitive Glucose Fluorescence Sensors, ideally suited for physiological glucose determination in blood, tears, interstitial fluid, serum or aqueous humor.

Our Glucose Probes readily chelate glucose, and are able to analytically determine glucose in an intensity manner, readily responding to glucose in < 1- 200 mM range. Our Mellitus Violet™ and Mellitus Blue™ class of Glucose Probes are ideally suited to determine tear glucose levels, with responses below 2 mM glucose.

Ursa offers 6 Fluorescence Intensity Probes and 2 Control Sample Probes for glucose detection and determination. Our Mellitus Violet™ 1-3 probes, can be readily excited at 320 nm, with emission around 430 nm. The pKa values of Mellitus Violet™ 1-3 in the presence of 100 mM glucose are 6.10, 6.85 and 6.95 respectively and 5.00, 5.05 and 5.45 for 100 mM fructose.

ProbeTech Glucose Fig 1 w600 h200

Figure 1. (A) Absorption and emission spectra recorded for Mellitus Violet 1™. (B) Absorption and emission Spectra recorded for Mellitus Blue 1™. The emission spectra is shown for two different excitation wavelengths, 1. excitation at 320 nm and 2. excitation at 345 nm.

 

Our Mellitus Blue™ 1-3 fluorescence intensity probes are highly sensitive to glucose and can readily be excited in the 300-400 nm range, with fluorescence emission centered around 460 nm. The pKa values of Mellitus Blue™ 1-3 in the presence of glucose are 6.62, 6.90 and 6.90 respectively and 4.80, 5.00 and 5.45 for 100 mM fructose.

Emission Spectra of Mellitus Blue 1™

Figure 3. Emission Spectra of Mellitus Blue 1™ in pH 7.5 phosphate buffer with increasing concentrations of Glucose.

Emission Spectra of Mellitus Violet 1™

Figure 4. Emission Spectra of Mellitus Violet 1™ in pH 7.5 phosphate buffer with increasing concentrations of Glucose.

 

Glucose Control Compounds

Ursa BioScience also offers two control compounds for both the Mellitus Blue™ and Violet™ Fluorescent Probes, namely Mellitus Blue C™ and Mellitus Violet C™ respectively. While both Control Compounds have the same excitation and emission wavelengths as their respective parent compounds, they lack the ability to chelate glucose, making them ideal for researchers wishing to study the potential influence of other analytes on the fluorescence properties of the glucose-chelating dyes, without switching wavelengths, filters etc.

 

 

 

Contour emission graph recorded for Mellitus Blue 3™

Contour emission graph recorded for Mellitus Blue 3™ dissolved in water.

 

Contour emission graph recorded for Mellitus Blue 1™ dissolved in water.

Contour emission graph recorded for Mellitus Blue 1™ dissolved in water.

 

Glucose probes are packed in standard vials

Glucose probes are shipped in standard vial packing for easy reconstitution.