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pH Probes

Ursa BioScience offers a water/MeOH soluble absorption (colorimetric) and fluorescence emission wavelength ratiometric pH Probe, which responds over the pH range 6-8, making it ideal for physiological measurements, such as for cellular studies, imaging and microscopy.

Amino Blue™ shows an absorption wavelength ratiometric response to pH in the 325 – 450 nm range and a fluorescence emission wavelength ratiometric response from 400-700 nm.

Ursa BioScience also offers a Control pH Fluorescent Compound for Amino Blue™. Our Amino Blue C™ has the same chromophore fluorescent backbone, but is only very mildly influenced by pH. For researcher’s and physicians alike undertaking pH analysis, Amino Blue C™ allows for any background interference of the parent fluorophore Amino Blue™ to be corrected for, while not changing any fluorescence instrumentation or filter settings. Subsequently, Amino Blue C™ does not show any ratiometric response to pH measurement. Amino Blue C™ can readily be excited from 375-450 nm, with an emission centred at 560 nm. Both the parent and control compounds have quantum yields ≈ 0.5.

Our pH probes are sold dry and are simply reconstituted by adding water/MeOH. One vial of our probe will typically allow for 10 separate pH measurements to be undertaken, when further diluted into 3 cm3 volumes within a cuvette.

Read more about our pH probes in our technology section.

 

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pH Probes

Amino Blue C™ is the control...

Sales price: $150.00

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Amino Blue™ is a modestly water soluble...

Sales price: $299.00

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