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Rotational Blue 1™

Similar to Ursa Blue™, but less polar and water soluble, Rotational Blue 1™ is ideally suited for a wide variety of fluorescence-anisotropy based particle sizing applications and depending on the polarity of the bulk phase and nanoparticle surface, can partition itself accordingly.
Rotational Blue 1™Rotational Blue 1™

Price:
Sales price: $399.00

Description

Similar to Ursa Blue™, but less polar and water soluble, Rotational Blue 1™ is ideally suited for a wide variety of fluorescence-anisotropy based particle sizing applications and depending on the polarity of the bulk phase and nanoparticle surface, can partition itself accordingly. In colloidal silica nanoparticle solutions, Rotational Blue 1™ shows triple exponential fluorescence decay with mean lifetimes of 16.1, 18.6 and 17.5 ns for DuPont’s SM30, AM30 and AS40 respectively. Rotational Blue 1™ is a cationic dye, which can readily be excited 300-400 nm, with an emission centered ≈ 445 nm.

Rotational Blue 1™ is sold dry and is simply reconstituted by adding MeOH or EtOH. One vial of Rotational Blue™ will typically allow for 10 nanoparticle metrology measurements to be undertaken, when further diluted into 3 cm3 volumes within a cuvette. 50 µL of MeOH/EtOH is typically added to each dry vial. Subsequently, 5 µL aliquots diluted to 3 cm3, typically give an optical density at 350 nm of ~0.1 OD. Note: that the partitioning of Rotational Blue between the nanoparticle surface and the bulk solvent phase is a function of the nanoparticle charge / polarity, bulk phase hydrophilicity / polarity.

For optimum performance, probe vials should be stored at -2 to -8 C when not being used. Probe solutions should be used after dilution.

An Ursa BioScience short video describes our reconstitution procedure.

Read more about Ursa BioScience's Anisotropy probes in the technology section.                                       Rotational Blue 1™ MSDS  material-safety-data-sheets-msdsthump