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Stay-Brite™

We offer a unique, ready to use, Anti-Fade Solution, Stay-Brite™, to protect against the photobleaching of fluorophores across the visible spectrum.  Our non-toxic solution can readily be added to virtually any fluorophore solution at room temperature to enhance the photostability, enabling you to use either higher light / laser intensities (irradiances) or even study your samples for much longer.  Our Stay-Brite™ solution is unique, an ideal addition to your every microscopy experiment.  Stay-Brite™ is not available anywhere else and can be stored on the bench at room temperature.

Figure 1A shows the intensity decay of Acriflavin, a common fluorophore, from both water and Ursa BioSciences’ Stay-Brite™ Anti-Fade Solution.  The Acriflavin, while rapidly photobleached in water, is much brighter in the Stay-Brite™ solution, and for a longer period of time.  In addition, the absorption spectrum of Acriflavin in 100% Stay-Brite™ solution is unperturbed, remaining the same in both water and 100 % Stay-Brite™, Figure 1B.

Similarly, Rhodamine 110 in both water and 100 % Stay-Brite™, shows a significantly enhanced photostability when excited at 473 nm, 17 mW, Figure 1C.

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Figure 1. (A) Intensity decays observed for Acriflavin dissolved in Stay-Brite™ (red line) and in water (blue line). The exciation wavelength was 474 nm. (B) Absorbance spectra recorded for Acriflavin dissolved in Stay-Brite™ (red line) and in water (blue line). (C) Intensity decays recorded for Rhodamine 110 chloride dissolved in Stay-Brite™ (red line) and in water (blue line).

 

Stay-Brite™ is a visually almost colorless (very slight yellow tint) non-toxic solution with a viscosity very similar to that of water.  Stay-Brite™ is ideal when used in the wavelength range 400 – 850 nm and be stored at room temperature on the bench.  The refractive index at 20 C is ≈ 1.3373 with a surface tension of ≈ 57.5 dynes/cm.

 

Protein-Brite™

It is well-known that the photo bleaching of fluorescent proteins in response to prolonged exposure to exciting radiation significantly impacts their performance as in vivo labels.  It has been shown that oxidative reddening of green fluorescent proteins is one of the main sources of GFP photo bleaching. Subsequently, Ursa BioScience has developed, Protein-Brite, a solution additive, which can be added to culture media 15 mins before imaging to enhance fluorescent protein photostability.  We recommend the use of Protein-Brite with our Rhacostoma GFP as well as our GFPs from Aequorea victoria.

Protein-Brite™ is sold in convenient 5 and 10 ml volumes with dropper bottles, and should be stored between 1-3 °C.

Protein-Brite - enhance your signal

Figure 2. Absorption spectra recorded for Protein-Brite™ in a standard 1 cm quartz cuvette.

 

Fade-Away™

We also offer a rapid Photo-Fade Solution, Fade-Away™, which is non-toxic and rapidly bleaches unwanted both color and fluorescence emission from fluorophores.  The Fade-Away™ solution is a unique solution and is not offered elsewhere.  Fade-Away™ is ideal for the rapid bleaching of color/fluorescence in microscopy applications, such as the rapid quenching of extra-cellular unwanted fluorescence, or the bleaching of excess fluorescence labels.

Fade-Away™ can be applied to many fluorophore solutions.  Figure 3A shows both the absorbance and fluorescence emission spectrum of the popular fluorophore Rose Bengal.  After only 30 seconds, both the absorption and fluorescence spectrum have almost completely photo-faded.  Similarly, both the absorption and emission of Eosin Y are significantly reduced after only a few seconds in the Fade-Away™ solution, Figure 3B.

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Figure 3. (A) Absorbance (solid line) and fluorescence (dotted line) spectra recorded for Rose bengal dissolved in Fade-Away™ (red) and in water (blu) line. (B) Absorbance (solid line) and fluorescence (dotted line) spectra recorded for Eosin Y dissolved in Fade-Away™ (red) and in water (blu) line. (C) Comparision of intensity decays recordede for Acriflavin dissolved in Stay-Brite™ (red line) and Fade-Away™ (blue line). The exciation wavelength was 474 nm.

 

It is also interesting to compare the intensity decay of Acriflavin in both Stay-Brite™ and Fade-Away™, Figure 2C.  In stay-Brite™ the fluorescence intensity slowly decays over time, as compared to Fade-Away™, where within 20 seconds the Acriflavin fluorescence has significantly diminished.

Fade-Away™ is a visually colorless non-toxic solution with a viscosity very similar to that of water (1.07 cP at 20 C).  Fade-Away™ is ideal when used in the wavelength range 360 – 850 nm and can be stored at room temperature on the bench.  The refractive index is 1.3331 @ 20 C, a solution osmolality of 3 mOsms/L, with a surface tension is ≈ 72 dynes/cm.

 

Ursa BioScience’s Pledge to Green ChemistryECO

For added convenience to our customers and to reduce the impact on our environment, our Stay-Brite™ and Fade-Away™ products do not need to be stored cold.  Our reagents can be kept on the bench.  In addition, our products are NOT formulated with the highly toxic sodium azide, like other anti-fade solutions, significantly helping to minimize environmental and health hazard exposure risks.

 

 

 

 

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