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Rabbit-derived antibodies to Native Jellyfish GFP

Ursa BioScience offers a range of Anti-Native GFP rabbit-derived Antibodies with different purities.

At a glance 
Ammonium Sulfate Fractionated Anti-GFP Purity: > 70 % by HPLC
Protein A Purified Anti-GFP Purity: >99% by SDS-PAGE & HPLC
Affinity Purified Anti-GFP Purity: >99% by SDS-PAGE & HPLC
Non Affinity Purified Anti-GFP Purity: 94 % by SDS gel

 

Ammonium Sulfate Fractionated Anti-Native GFP Polyclonal Antibody, (UB-GFPab2)

Specificity: Ursa BioScience offers an Ammonium Sulfate fractionated polyclonal antibody raised in rabbits against highly-purified native GFP from Aequorea victoria. It is reactive with GFP and GFP fusion proteins from both native and recombinant sources. This anti-GFP recognizes all variants of A. victoria GFP induced in transformed organisms, including all vertebrate species tested. Figure 1A shows the specificity of our Anti-Native GFP.

Figure 1 2

Figure 1. (A) Specificity of our Ammonium Sulfate Fractionated Anti-Native GFP Polyclonal Antibody, (UB-GFPab2). Lane 1 – Protein standards; Lane 2 – EGFP (Partially Purified); Lane 3 – BFP (Partially Purified); Lane 4 – E. coli. expressing wild-type rGFP; Lane 5 – S65T GFP (Partially purified); Lane 6 – DsRed (Partially Purified). (B) Purity Grades of the Rabbit Anti-Native GFPs we offer. Lane 2 (first lane) – UB-GFPab2 (Ammonium Sulfate Fractionated); Lane 3 – UB-GFPab3 (Protein A purified); Lane 4 – UB-GFPab4 (Affinity Purified); Lane 5 – Blank; Lane 6 – Bio Rad Protein standards.

 

Immunogen: Ursa BioScience is unique among other anti-GFP suppliers, in that we use highly purified native GFP from Aequorea Victoria. Because our immunogen is natural rather than recombinant, commercial application of our antibodies requires no license. Purified GFP, a 27 kDa monomer consisting of 238 amino acids, emits green light (emission maximum at 509 nm) when excited with blue or UV light. The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins.

Applications: Immunoblotting: 1:1000-1:5000 dilution, using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods. ELISA: 1:2,000 – 1:10,000.

Titer: UB-GFPab2 titers to 1/10,000,000 using an alkaline phosphate and nitroblue tetrazolium assay.

Purity: > 70% by HPLC. Figure 1B (Lane 2) shows the purity grades of the Rabbit Anti-GFPs we offer.

We offer our Ammonium Sulfate Fractionated Anti-Native GFP Polyclonal Antibody, (UB-GFPab2) in 200 µg quantities.

Protein A Purified Anti-Native GFP Polyclonal Antibody, (UB-GFPab3)

Specificity: UB-GFPab3 is a Protein-A purified polyclonal antibody raised in rabbits against highly-purified native GFP from Aequorea victoria. It is reactive with GFP and GFP fusion proteins from both native and recombinant sources. This anti-GFP recognizes all variants of A. victoria GFP induced in transformed organisms, including all vertebrate species tested.  Figure 2A shows the specificity of our Anti-Native GFP.

Immunogen: Ursa BioScience is unique among other anti-GFP suppliers, in that we use highly purified native GFP from Aequorea Victoria. Because our immunogen is natural rather than recombinant, commercial application of our antibodies requires no license. Purified GFP, a 27 kDa monomer consisting of 238 amino acids, emits green light (emission maximum at 509 nm) when excited with blue or UV light. The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins.

Applications: Immunoblotting: 1:1000-1:5000 dilution, using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods. ELISA: 1:2,000 – 1:10,000

Titer: UB-GFPab3 titers to 1/10,000,000 using an alkaline phosphate and nitroblue tetrazolium assay.

Purity: > 99% by SDS-PAGE & HPLC. Figure 1B (Lane 3) shows the purity grades of the Rabbit Anti-GFPs we offer.

We offer our Protein A Purified Anti-Native GFP polyclonal Antibody in two quantities, 100 µg and 1 mg. Larger quantities are available on request.

Figure 2

Figure 2. (A) Specificity of our Protein A Purified Anti-Native GFP Polyclonal Antibody, (UB-GFPab3). Lane 1 – Protein standards; Lane 2 – EGFP (Partially Purified); Lane 3 – BFP (Partially Purified); Lane 4 – E. coli. expressing wild-type rGFP; Lane 5 – S65T GFP (Partially purified); Lane 6 – DsRed (Partially Purified). (B) Specificity of our Affinity Purified Anti-Native GFP Polyclonal Antibody, (UB-GFPab4). Lane 1 – Protein standards; Lane 2 – EGFP (Partially Purified); Lane 3 – BFP (Partially Purified); Lane 4 – E. coli. expressing wild-type rGFP; Lane 5 – S65T GFP (Partially purified); Lane 6 – DsRed (Partially Purified).

 

Affinity-Purified Anti-Native GFP Polyclonal Antibody, (UB-GFPab4)

Specificity: Ursa BioScience offers an affinity-purified polyclonal antibody (UB-GFPab4) raised in rabbits against highly-purified native GFP from Aequorea victoria. It is reactive with GFP and GFP fusion proteins from both native and recombinant sources. This anti-GFP recognizes all variants of A. victoria GFP induced in transformed organisms, including all vertebrate species tested. Figure 2B shows the specificity of our Affinity Purified Anti-Native GFP.

Immunogen: Ursa BioScience is unique among other anti-GFP suppliers, in that we use highly purified native GFP from Aequorea Victoria. Because our immunogen is natural rather than recombinant, commercial application of our antibodies requires no license. Purified GFP, a 27 kDa monomer consisting of 238 amino acids, emits green light (emission maximum at 509 nm) when excited with blue or UV light. The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins.

Applications: Immunoblotting: 1:1000-1:5000 dilution, using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods. ELISA: 1:2,000 – 1:10,000.

Titer: UB-GFPab4 titers to 1/10,000,000 using an alkaline phosphate and nitroblue tetrazolium assay.

Purity: >99% by SDS-PAGE & HPLC. Figure 1B (Lane 4) shows the purity grades of the Rabbit Anti-GFPs we offer.

We offer our Affinity-Purified Anti-Native GFP polyclonal Antibody in two quantities, 100 µg and 1 mg. Larger quantities are available on request.

Affinity-Free Anti-Native GFP Polyclonal Antibody, (UB-GFPab5)

Specificity: Ursa BioScience supplies a non-affinity method for purifying both rabbit IgG and goat IgG. We refer to the method as “A-free-IgG” as no affinity technique is needed to provide up to 99% pure IgG from polyclonal antiserum from rabbits. UB-GFPab5 is our “A-free” polyclonal antibody raised in rabbits against highly-purified native GFP from Aequorea victoria. It is reactive with GFP and GFP fusion proteins from both native and recombinant sources. This anti-GFP recognizes all variants of A. victoria GFP induced in transformed organisms, including all vertebrate species tested. Figure 3A shows the specificity of our non-Affinity Purified Anti-Native GFP.

Immunogen: Ursa BioScience is unique among other anti-GFP suppliers, in that we use highly purified native GFP from Aequorea Victoria. Because our immunogen is natural rather than recombinant, commercial application of our antibodies requires no license. Purified GFP, a 27 kDa monomer consisting of 238 amino acids, emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Applications: Immunoblotting: 1:1,000- 1:5,000 dilution, using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods. ELISA: 1:2,000 – 1:10,000.

Titer: UB-GFPab5 titers to 1/10,000,000 using an alkaline phosphatase and nitroblue tetrazoliumassay.

Purity: 94 % by SDS gel. Figure 3B shows the Purity of our Affinity-free Anti-Native GFP from SDS-PAGE.

We offer our non  Affinity-Purified Anti-Native GFP polyclonal Antibody in two quantities, 100 µg and 1 mg. Larger quantities are available on request.

Figure 3

Figure 3. (A) Specificity of our non-Affinity Purified Anti-Native GFP Polyclonal Antibody, (UB-GFPab5). Lane 1 – Protein standards (kDa); Lane 2 – 10 µg wt- E. coli lysate; Lane 3 - 10 µg E. coli w/GFP; Lane 4 - 1 µg E. coli w/GFP; Lane 5 - 100 ng E. coli w/GFP: Western: 1/5000 UB-GFPab5 + 1/5000 goat anti-rabbit AP conjugate. (B) Purity of our non-affinity (A-free IgG) purified Anti-GFP using SDS-Page. Lane 1 – Protein standards; Lane 2 – 2 µg of Protein A purified Anti-GFP.

 

Background on GFP

Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein-protein interactions through the yeast two hybrid system and measurement of distance between proteins through Forster Resonance energy Transfer (FRET) protocols. GFP is used to measure single cell metastasis and successful proliferation of stem cells.

Visit of wt-GFP technology page to learn more about the GFPs we offer.

 

 

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